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LAMP1-SEP-mRuby3

Plasmid

#200939

Purpose

endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

LAMP1 (LAMP1 Human)
SEP-mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBZ-mRuby2

Plasmid

#110650

Purpose

expresses a PAR-binding (PBZ) domain fused to mRuby2

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ChRmine-mRuby2-ST

Plasmid

#227777

Purpose

Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChRmine-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA1-mRuby3

Plasmid

#184888

Purpose

Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA1 (ANXA1 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ChR2-mRuby2-ST

Plasmid

#109125

Purpose

Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChR2-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-mRuby3-FTH1

Plasmid

#192804

Purpose

Stable expression of mRuby3-FTH1 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

FTH1 (FTH1 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1

Plasmid

#172587

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Use

Tags

Lifeact-mRuby and mRuby

Expression

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

Plasmid

#124903

Purpose

Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

p65 promoter- p65 CDS-mRuby2

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-ChroME-mRuby2-ST

Plasmid

#108902

Purpose

Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ChrimsonR-mRuby2-ST

Plasmid

#105447

Purpose

Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

ChrimsonR-mRuby2-ST

Use

Tags

mRuby2 and soma targeting motif

Expression

Mammalian

Mutation

K176R in Chrimson

Promoter

pCAGGS

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAX

Plasmid

#111628

Purpose

Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Use

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-mRuby2

Plasmid

#49089

Purpose

Clover-mRuby2 tandem fusion for FRET calibration

Depositor

Kurt Beam

Insert

Clover-mRuby2 fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CMV mRuby3-Synapsin1a

Plasmid

#187896

Purpose

For labeling pre-synaptic terminals

Depositor

Timothy Ryan

Insert

Synapsin1a

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA5-mRuby3

Plasmid

#184891

Purpose

Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA5 (ANXA5 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge