We narrowed to 928 results for: multisite gateway

1. pME-mScarlet3-Giantin (JDW 1356)

   Plasmid

   #224502

   Purpose

   Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet3-Giantin
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME EFS-rtTA/rtTA3 (JDW 1230)

   Plasmid

   #224525

   Purpose

   A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS-rtTA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME Actin Vhh Halo Tag (JDW 1223)

   Plasmid

   #224498

   Purpose

   Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-Halo-Tag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-DRE-nls (JDW 31)

   Plasmid

   #242561

   Purpose

   Gateway middle entry clone containing Dre recombinase

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   codon optimized DRE-nls
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-EGFP-miR-MCS (JDW 1231)

   Plasmid

   #242562

   Purpose

   Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP with artifial intron and MCS
   

   Use

   Gateway subcloning

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Lifeact-mRuby2-3xMyc (JDW 1246)

   Plasmid

   #242554

   Purpose

   Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mRuby2
   

   Use

   Gateway subcloning

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-zFUCCI (JDW 1464)

   Plasmid

   #242558

   Purpose

   Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-H2B-mStayGold-WPRE (JDW 1384)

   Plasmid

   #242550

   Purpose

   Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mStayGold (monomeric StayGold)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-H2B-mBaoJin-WPRE (JDW 1383)

    Plasmid

    #242549

    Purpose

    Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mBaoJin
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-H2B-mScarlet-3H (JDW 1513)

    Plasmid

    #242551

    Purpose

    Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-H
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-mCerulean3-TUBB1A (JDW 1508)

    Plasmid

    #242555

    Purpose

    Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean3-TUBB1A
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME nls BFP FLAG WPRE bGH pA (JDW 484)

    Plasmid

    #224539

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mTagBFP2-FLAG_WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-WPRE-bGH-pA (JDW 1221)

    Plasmid

    #224527

    Purpose

    A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-bGH
    

    Use

    Gateway cloning

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

    Plasmid

    #224486

    Purpose

    Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2B-V5-n2StayGoldC4
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME V5 mScarlet-I (JDW 1323)

    Plasmid

    #224487

    Purpose

    Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-myr-mTagBFP2-Flag (JDW 1183)

    Plasmid

    #224492

    Purpose

    Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    myr-mTagBFP2-Flag
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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