Addgene

We narrowed to 894 results for: multisite gateway

Showing: 161 - 180 of 894 results

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mClover3-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-mTagBFP2-pA (JDW 1361)

Plasmid

#224535

Purpose

A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG-NLS-mTagBFP2-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-mmCdh5pro (JDW 1239)

Plasmid

#224478

Purpose

Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Cdh5 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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p5E TRE-3GV (JDW 1163)

Plasmid

#224476

Purpose

Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

TRE3GV promoter

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

attL1/L2 flanked MCS

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

CAGGS Promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p5E_hs_Glast (JDW 1182)

Plasmid

#224483

Purpose

Gateway compatible 5' entry clone containing the Human GLAST promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

GLAST promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC (no LexOp) (JDW 937)

Plasmid

#224504

Purpose

Gateway compatible middle entry clone containing a destabilized LexA transactivator

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexA-mODC

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mTagBFP2 (JDW 871)

Plasmid

#224531

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mTagBFP2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-ChBP

Plasmid

#108892

Purpose

Multisite gateway vector for 3' tagging with a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

ChBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS715

Plasmid

#188887

Purpose

Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-nls

Plasmid

#108882

Purpose

Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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