We narrowed to 4,504 results for: pcr

1. pCRY2PHR-mCherryN1

   Plasmid

   #26866

   Purpose

   Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2PHR (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR4-24XPP7SL

   Plasmid

   #31864

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPR-cBEST

   Plasmid

   #125689

   Purpose

   C to T base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Insert

   streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR-mcBEST

   Plasmid

   #209416

   Purpose

   Plasmid for multiplexed cytosine base editing in streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

   Inserts

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   csy4 from Pseudomonas aeruginosa PAO1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   base editing cassette: PtipA ; sgRNAs: PkasO* an…

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPEY-Z3-Nat

   Plasmid

   #232100

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR2.1-InC

   Plasmid

   #83959

   Purpose

   Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

   Depositor

   Kai-Shun Chen

   Insert

   Internal Control sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR-aBEST

   Plasmid

   #131464

   Purpose

   Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Inserts

   Streptomyces codon optimized spCas9n (D10A)
   streptomyces codon optimized tadA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tipA

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR3.1-mIL2

    Plasmid

    #74467

    Purpose

    expression of mouse IL2 in mammalian cells

    Depositor

    Loree Heller

    Article

    Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

    Insert

    IL2 (Il2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcrDNA3.1-Chimera

    Plasmid

    #100891

    Purpose

    Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPaint-HA-PuroR

    Plasmid

    #80959

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    HA tag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCralbp-DsRed

    Plasmid

    #11158

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    Cralbp promoter (Rlbp1 Mouse)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcrDNA3.1-PRKACA

    Plasmid

    #100890

    Purpose

    Mammalian expression of PRKACA

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Isoform 1 wild type

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCruzHA SIRT1

    Plasmid

    #10962

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-ProteoTuner DD

    Plasmid

    #67181

    Purpose

    CRISPaint universal donor for fusion tagging allowing inducible destabilization

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    ProteoTuner DD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR3.1-OPNa

    Plasmid

    #107980

    Purpose

    expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    full-length osteopontin (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge