Addgene

We narrowed to 1,395 results for: tk

Showing: 161 - 180 of 1395 results

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SpCas9 iFE

Plasmid

#190138

Purpose

Expresses SpCas9-POLB (iFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9-POLB (HiFi iFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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1x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64390

Purpose

encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pG6L6 CAT (P#1541)

Plasmid

#14590

Depositor

Matija Peterlin

Article

Zhang et al Genes Dev. 2003 Mar 15. 17(6):748-58.

Insert

5xGal4 and 5xLexA binding sites

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAWSV-3

Plasmid

#72756

Purpose

pBT264-[let-7c]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

[let-7c]

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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LZ3 iFE

Plasmid

#190143

Purpose

Expresses LZ3Cas9-POLB (LZ3 iFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

LZ3Cas9-POLB (LZ3_iFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

Plasmid

#81246

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9 dFE

Plasmid

#190137

Purpose

Expresses SpCas9-T4pol (dFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9-T4pol (dFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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Construct 1 - U6p_20nt1_WT_sgRNA

Plasmid

#81245

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLuxR

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pIM04

Plasmid

#132584

Purpose

pZS2-pLsoxS-TEVp-proD-soxR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

pLsoxS-TEVp-TermT1
proD-soxR-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLsoxS and proD

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pCVD443

Plasmid

#66150

Purpose

cloning of EPEC E.coli eae gene

Depositor

James Kaper

Article

McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

Insert

eae

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pIM16

Plasmid

#132583

Purpose

pZA4-oxySp-mCherry-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
pLsoxS-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

oxySp and pLsoxS

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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12x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64393

Purpose

encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
TJF205

Plasmid

#132576

Purpose

pZE1-oxySp-oxyR-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

oxySp-oxyR-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

oxySp

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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