We narrowed to 219 results for: ube2s

1. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3-AUP1-FLAG-DCUE

   Plasmid

   #185339

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 293-337 corresponding to the CUE d…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6P-1-HRD1 aa 236-444

   Plasmid

   #185349

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   HRD1/SYVN (SYVN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 236-444 only

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-6P-1-gp78 RING

   Plasmid

   #185353

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   gp78 cytoplasmic domain (AMFR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Stop codon after aa 393

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-1-gp78 429-611

   Plasmid

   #185354

   Purpose

   GST fusion protein for quantification of gp78 levels

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 429-611 only

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-6P-1 AUP1 292-410 5E

   Plasmid

   #185332

   Purpose

   Bacterial expression of AUP1 with GST fusion protein for binding assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 292-410 only, with mutations R382E/Q383E/K390E…

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-6P-1 AUP1 292-410 A397D

   Plasmid

   #185333

   Purpose

   Bacterial expression of AUP1 with GST fusion protein for binding assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 292-410 only, with mutation A379D

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-C1-G2BRgp78

   Plasmid

   #185344

   Purpose

   Expression of soluble G2BR, amino acids 575-600 of human gp78

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   gp78 aa 574-600 (G2BR) (AMFR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 575-600 only

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSUMO3

   Plasmid

   #52260

   Purpose

   Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-3 (SUMO3 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO2

    Plasmid

    #52259

    Purpose

    Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 2ONU

    Plasmid

    #51346

    Depositor

    Cheryl Arrowsmith

    Insert

    2ONU
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. UBC53

    Plasmid

    #25471

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc53.001.150 (UBE2U Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAIP4_2ESK

    Plasmid

    #234232

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2ESK (UBE2D2 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. FLJ11011

    Plasmid

    #25612

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FLJ11011 (UBE2W Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pHAGE-mNeptune2-SKL

    Plasmid

    #183729

    Purpose

    For exrpression of mNeptune2 targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mNeptube2
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3/RGS-UBC12

    Plasmid

    #20080

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1999 Apr 23;274(17):12036-4

    Insert

    UBC12 (UBE2M Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pFlagCMV2-UbcH8

    Plasmid

    #12442

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    UbcH8 (UBE2L6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pET-UbcH10-wt

    Plasmid

    #8503

    Depositor

    Joan Ruderman

    Article

    Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

    Insert

    UbcH10 (UBE2C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2005

    Availability

    Academic Institutions and Nonprofits only

20. p3990 pET ubcH8

    Plasmid

    #8650

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH8 (UBE2L6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only