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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00681

Plasmid

#39726

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14513 (yemalpha Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetO-Chim-Salhel-I-K57E

Plasmid

#231519

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG K57E flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Point mutation at position 57 K to E of the HMG

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salro-Sox-like

Plasmid

#231531

Purpose

Expresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-like

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-acatenin

Plasmid

#186458

Purpose

Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET41SIII-ATF6(1-373)

Plasmid

#171074

Purpose

Expresses of ATF6α(1-373) in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

ATF6α (ATF6 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 AKT1

Plasmid

#67102

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Gal4pBS134-Pleum

Plasmid

#60339

Purpose

Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Flag-PGC1a-6His

Plasmid

#67637

Purpose

AAV vector expressing PGC1a gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

PGC1a (Ppargc1a Mouse)

Use

AAV

Tags

6XHis and Flag tag

Expression

Mutation

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pPW3758 : CMVd3-HsIRE1-HaloTag

Plasmid

#185680

Purpose

Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
pECE HA AMPKa1 WT

Plasmid

#69504

Purpose

Expresses AMPK alpha1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Human a4 nicotinic subunit crystallization construct

Plasmid

#85841

Purpose

pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

Depositor

Ryan Hibbs

Article

Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

Insert

Human a4 nAChR subunit crystallization construct (CHRNA4 Human)

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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