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pMTU-DO-LEU

Plasmid

#160006

Purpose

Low-copy expression vector for K. marxianus, leucine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pIII/MS2-2

Plasmid

#220630

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-600μΔ13V

Plasmid

#28001

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-600μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

F16A in mglB

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLII12Pglu-700μδ6

Plasmid

#28002

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLII12Pglu-700μδ6

Tags

eCFP and eYFP

Expression

Yeast

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-mCherry2Bx6

Plasmid

#105267

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Tags

mEGFP

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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VanR-wt

Plasmid

#153339

Purpose

Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

Depositor

Michael Jensen

Article

et al Biotechnology Notes, Volume 1, 2020, Pag

Insert

VanR

Expression

Yeast

Promoter

TEF1

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8644

Plasmid

#126919

Purpose

Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

msCFPx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LT1_33_pTEFmut8-mCherry

Plasmid

#177706

Purpose

Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEFmut8-mCherry

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGK401red-rtetTAK8N, L131L

Plasmid

#165968

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8787

Plasmid

#126921

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHES835

Plasmid

#87946

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Promoter

pZ

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8788

Plasmid

#126922

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEDJ333

Plasmid

#177272

Purpose

GAL1p:Cas9:CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

Cas9

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

TEF

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRSII42K

Plasmid

#194523

Purpose

High copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-HPH

Plasmid

#160014

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS10-msGFPx3

Plasmid

#105274

Purpose

Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps10 portion (PEP1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4170

Plasmid

#126750

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Promoter

pTRX2_5xUAS

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHHYTK113

Plasmid

#200144

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-URA

Plasmid

#160009

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-G418

Plasmid

#160007

Purpose

Low-copy expression vector for K. marxianus, G418 selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMS2-2

Plasmid

#220628

Purpose

Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHES840

Plasmid

#89191

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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