We narrowed to 12,367 results for: NUC

1. pJK644

   Plasmid

   #71629

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to aspartate

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK553

   Plasmid

   #72452

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene with ~40 silent substitutions

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK533

   Plasmid

   #72514

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, small subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains additional Ala2

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK575

   Plasmid

   #72526

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK620

   Plasmid

   #72537

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, small subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK622

   Plasmid

   #72538

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK648

   Plasmid

   #71611

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes phenylalanine-79 to tryptophan

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC21-deltaBB

   Plasmid

   #51657

   Purpose

   Bacterial cloning vector based on pUC21 (Amp)

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Py-U5-15kD

   Plasmid

   #25561

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Py-U5-15kD
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pGBW-m4134902

    Plasmid

    #151912

    Purpose

    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. RG6

    Plasmid

    #80167

    Purpose

    fluorescent protein splicing reporter

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Impact-T4gp43(exo-)

    Plasmid

    #228202

    Purpose

    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated aspartate 219 to alanine (D219A), elimina…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV:ECFP(FRT)EYFP

    Plasmid

    #31373

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    ECFP(FRT)EYFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHM840

    Plasmid

    #20701

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-NLS-lacZ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4-HA

    Plasmid

    #27553

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

    Type

    Empty backbone

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV:DsRed(FRT)GFP

    Plasmid

    #31128

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    DsRed(FRT)GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInt_attP1_LCS_kanR

    Plasmid

    #205300

    Purpose

    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP_1

    Plasmid

    #238192

    Purpose

    Empty lentiviral vector for expressing protein sequences fused to GFP, under a TRE3GV promoter

    Depositor

    Feng Zhang

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP and SV40

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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