We narrowed to 4,657 results for: lum

1. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-EGFP-Sec61β

   Plasmid

   #174466

   Purpose

   Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with endoplasmic reticulum-targeting sequence
   

   Tags

   Sec61 subunit beta

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMO746

   Plasmid

   #117480

   Purpose

   upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

   Depositor

   Judy Wall

   Article

   Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

   Insert

   uracil phosphoribosyltransferase
   

   Expression

   Bacterial

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-J6-NbGP41-sfGFP-VP64-GB1-J9

   Plasmid

   #202007

   Purpose

   MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   NbGP41-sfGFP-VP64-GB1
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GB1 and sfGFP

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Lifeact-mRuby

   Plasmid

   #194339

   Purpose

   Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Lifeact
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-303

   Plasmid

   #159678

   Purpose

   Gateway destination vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PERK 3: PERK.dC.9E10.pCDNA

   Plasmid

   #21816

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (C-term deleted) (Eif2ak3 Mouse)
   

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Mutation

   amino-acids 582-1081 deleted

   Available Since

   July 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAV1-K*R-HA

   Plasmid

   #27765

   Depositor

   Ari Helenius

   Article

   Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

   Insert

   caveolin-1, lysine null mutant (CAV1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   All lysines mutated to arginines (K*R)

   Available Since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

    Plasmid

    #202010

    Purpose

    Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GB1 and sfGFP

    Promoter

    CmYLCV

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

13. PERK 4: PERKKD-pGEX4T-1

    Plasmid

    #21817

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (537-1114) (Eif2ak3 Mouse)
    

    Tags

    GST and Myc (9E10)

    Expression

    Bacterial

    Mutation

    PERK Kinase Domain. Amino acids 537-1114.

    Available Since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. mCherry-TIS11B CPM2

    Plasmid

    #121143

    Purpose

    Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

    Plasmid

    #134863

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SAC1C389SdeltaTMD-FKBP-mCherry

    Plasmid

    #108124

    Purpose

    Recruitable cytosolic inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    SACM1L(C389S; 1-520)

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAV1-mEGFP-mCherry

    Plasmid

    #27763

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric EGFP-monomeric Cherry (tandem)

    Expression

    Mammalian

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-VHL W117R-pBabe-puro

    Plasmid

    #19239

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tryptophan 117 to Arginin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCaRNA3-eIFiso4E120(+)

    Plasmid

    #86092

    Purpose

    This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    eIFiso4E
    

    Expression

    Plant

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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