We narrowed to 4,631 results for: lum

1. HA-VHL W117R-pBabe-puro

   Plasmid

   #19239

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tryptophan 117 to Arginin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-UFM1 sgRNA2

   Plasmid

   #134634

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA2 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-UFM1 sgRNA1

   Plasmid

   #134633

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA1 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETconNK-TEM1(S70A,D179G)

   Plasmid

   #81166

   Purpose

   pETconNK with TEM1(S70A,D179G) beta-lactamase

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   GAL1 and gal1

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

   Plasmid

   #22132

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   synthethic oligonucleotide
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   synthethic oligonucleotide encoding shRNA targeti…

   Promoter

   Available since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGFAP R239C

   Plasmid

   #169476

   Purpose

   Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed R 239 to C

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbR-HIS-Xyl-DocI

   Plasmid

   #58711

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Xyl-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Threonin 120 to Cysteine in Xylanase Doma…

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-FRISZ-TM

   Plasmid

   #176886

   Purpose

   AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

   Depositor

   Huiwang Ai

   Article

   Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

   Insert

   Ig kappa-FRISZ-PDGFR TM
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTH PKC zeta

    Plasmid

    #8414

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mADAM17deltaMP in pQCXIP cFLAG

    Plasmid

    #19144

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 189-378

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

13. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRZ-mNanog-YNL

    Plasmid

    #65717

    Purpose

    mNanog reporter plasmid (expresses YNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Nanog promoter and YNL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

    Plasmid

    #192406

    Purpose

    To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Turbo GFP
    

    Use

    Synthetic Biology

    Tags

    N7

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

    Plasmid

    #173073

    Purpose

    Dead SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    AA144 in both SNAP tags changed from a cysteine t…

    Promoter

    CMV IE94

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV Gab1(PRD)-mCherry-HOTag3

    Plasmid

    #190026

    Purpose

    Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    Gab1(PRD) CluMPS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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