We narrowed to 10,632 results for: t7

1. WARS_pNIC-CTB10H

   Plasmid

   #153058

   Purpose

   Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   WARS (WARS1 Human)
   

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET11a_PTK2b_Kinase

   Plasmid

   #224242

   Purpose

   Expression of human PTK2b Kinase domain in E. coli

   Depositor

   Andrew Mesecar

   Insert

   ptk2b_kinase (PTK2B Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NUDT1

   Plasmid

   #74660

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT1 (NUDT1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. TC9pAB

   Plasmid

   #71310

   Purpose

   used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9
   

   Use

   In vitro transcription

   Tags

   NLS

   Promoter

   T7

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pet15b_ack

   Plasmid

   #220229

   Purpose

   Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   Acetate kinase (ackA )
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET15b/GgVcl 884-1066-ECFP

   Plasmid

   #46182

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Tags

   ECFP and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. TBXTA-c006

   Plasmid

   #139755

   Purpose

   For protein expression/crystallography (PDB:6F59)

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, G177D (TBXT Human)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids E41-S224

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pet15_rbsk

   Plasmid

   #220227

   Purpose

   Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   ribokinase (rbsK E.coli)
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET3a-DJ1

   Plasmid

   #51489

   Purpose

   Inducible expression of human DJ1 in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   DJ1 (PARK7 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PAHA

    Plasmid

    #74663

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKU1 (PAH Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. SPIN4

    Plasmid

    #73239

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN4 (SPIN4 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBbi-Pur-AktAR2

    Plasmid

    #125195

    Purpose

    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    AktAR2
    

    Use

    Transposon

    Tags

    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

    Plasmid

    #30185

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK408

    Plasmid

    #72422

    Purpose

    Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag

    Depositor

    T. J. Kappock

    Insert

    feruloyl esterase
    

    Tags

    His5

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK266

    Plasmid

    #73326

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    violacein biosynthesis protein VioD
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK367

    Plasmid

    #72406

    Purpose

    Produces Acetobacter aceti 1023 Est06

    Depositor

    T. J. Kappock

    Insert

    lipase
    

    Expression

    Bacterial

    Mutation

    lacks Met1 and Thr2 in annotated (v1) sequence

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ScSpe1_pET20b(+)

    Plasmid

    #118343

    Purpose

    Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains

    Depositor

    Markus Ralser

    Insert

    SPE1
    

    Tags

    Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK409

    Plasmid

    #72423

    Purpose

    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK405

    Plasmid

    #72418

    Purpose

    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge