We narrowed to 3,712 results for: mak

1. pHS1184

   Plasmid

   #205965

   Purpose

   NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH
   

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ARH2-c003

   Plasmid

   #173094

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRHL1 (ADPRHL1 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-K354, codon optimized

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-Chronos-mT-Sapphire-NRN

   Plasmid

   #149692

   Purpose

   For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   Chronos
   

   Use

   AAV

   Tags

   mT-Sapphire

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MDO2-c004

   Plasmid

   #173104

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K7-D243

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-Ac-AChBP

   Plasmid

   #63128

   Purpose

   Expression of Aplysia californica AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMRXIP 3xMyc-STX17ΔNTD

   Plasmid

   #89940

   Purpose

   Expresses syntaxin17 lacking N-terminal domain tagged with Myc in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 lacking N-terminal domain (STX17 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   deleted amino acids 1-161

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdKatushka2-VASP-5

   Plasmid

   #56049

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Insert

   VASP
   

   Tags

   tdKatushka2

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDR111_Bgal NLS

   Plasmid

   #188396

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Promoter

   Phyper spank

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IB-mGFP-FTL

   Plasmid

   #192801

   Purpose

   Stable expression of mGFP-FTL in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   FTL (FTL Human)
   

   Tags

   monomeric EGFP (A206K)

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS1398

    Plasmid

    #205971

    Purpose

    MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1182

    Plasmid

    #205964

    Purpose

    NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

    Plasmid

    #149693

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSP1D1R1

    Plasmid

    #97323

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1R1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of R residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. XRCC1-c003

    Plasmid

    #173090

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E315-P406

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FKBP-GFP-OPTN (2-119)

    Plasmid

    #208867

    Purpose

    Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    aa 2-119 only

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FH-TET3-HxDmut-pEF

    Plasmid

    #127896

    Purpose

    Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    TET3-HxDmut (TET3 Human)
    

    Tags

    HA and Flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T1-MBP-TEV-NAP1 (WT)

    Plasmid

    #208871

    Purpose

    For expression in E. coli of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-STX17TMΔC

    Plasmid

    #221737

    Purpose

    Stable expression of GFP-STX17TMΔC in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    STX17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-228 and 227-302

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDF0044 CjcCas7-11 full locus with synthetic array

    Plasmid

    #172502

    Purpose

    Encodes the CjcCas7-11 full locus for bacterial expression

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 full locus with synthetic array
    

    Expression

    Bacterial

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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