Addgene

We narrowed to 14,722 results for: nans

Showing: 1801 - 1820 of 14722 results

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pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-8

Plasmid

#228990

Purpose

For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-8

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL-HAx2-TEADiv2

Plasmid

#228873

Purpose

Expression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL-EGFP-TEADiv2

Plasmid

#228872

Purpose

Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-KaNLuc

Plasmid

#208855

Purpose

Expresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

KaNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-NpSxph

Plasmid

#193097

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NpSxphI559Y

Plasmid

#193099

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin I559Y

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

I559Y

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-dKeNLuc

Plasmid

#208856

Purpose

Expresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-CeNLuc

Plasmid

#208838

Purpose

Expresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNBA001

Plasmid

#155323

Purpose

Binary vector for expression of PULSE-controlled EFR-GFP

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNBA002

Plasmid

#155324

Purpose

Binary vector for expression of PULSE-controlled GBP-mCherry

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pROF018

Plasmid

#155325

Purpose

Constitutive expression of KRAB-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-NLS-KRAB-EL222-Tnos

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pROF021

Plasmid

#155326

Purpose

Expression of PULSE-controlled FLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-FLuc-TSV40

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pROF050

Plasmid

#155327

Purpose

Constitutive expression of BRD-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-BRD-NLS-EL222-Tnos

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge