We narrowed to 6,502 results for: poly

1. UtB2N7

   Plasmid

   #161015

   Purpose

   ubiquitously expressed nuclear localized tagBFP2

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   tagBFP2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyLigase

   Plasmid

   #51722

   Purpose

   Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

   Depositor

   Mark Howarth

   Article

   Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

   Insert

   SpyLigase
   

   Use

   Tags

   His6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AviTag-SpyCatcher

   Plasmid

   #72326

   Purpose

   Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   AviTag-SpyCatcher
   

   Use

   Tags

   N-term His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. LifeAct-scramble

   Plasmid

   #217499

   Purpose

   Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

   Depositor

   Erik Dent

   Article

   Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

   Insert

   LifeAct-mScarlet-scramble
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPINS-UBE3C

   Plasmid

   #66711

   Purpose

   Expresses UBE3C HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KiAA0010 (UBE3C )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLA230

   Plasmid

   #11724

   Depositor

   Lawrence Loeb

   Article

   Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

   Insert

   b-lactamase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GAA for Glu26 changed to TAA (premature stop codo…

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN3-3xFlag-Control

   Plasmid

   #107717

   Purpose

   Control expression plasmid for 3xFLAG-tagged pN3-contructs

   Depositor

   Guntram Suske

   Article

   Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

   Type

   Empty backbone

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. hTRAF2pLPCX-HA-Flag/P874

   Plasmid

   #20229

   Depositor

   Martin Dorf

   Article

   Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

   Insert

   TNF receptor-associated factor 2 (TRAF2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   unmutated NM_021138.3. L replaces M at beginning…

   Promoter

   Available since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-SENP2m

   Plasmid

   #18713

   Depositor

   Edward Yeh

   Article

   Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R577L, K578M

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

10. mFGF8b

    Plasmid

    #22090

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-RIP

    Plasmid

    #11972

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    RIP (Ripk1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGL2B -938/+64

    Plasmid

    #24943

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

13. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHpgRNA13

    Plasmid

    #160127

    Purpose

    To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-ADE2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-CDX2-WT

    Plasmid

    #16553

    Depositor

    Bert Vogelstein

    Article

    da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

16. AL13Rb-35S

    Plasmid

    #161008

    Purpose

    PP7-tagged reporter driven by the 35S promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMF2

    Plasmid

    #112819

    Purpose

    Expresses wild-type Rpb1 in yeast cells

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-KM

    Plasmid

    #20332

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Klf4-F2A-cMyc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UPG

    Plasmid

    #161003

    Purpose

    ubiquitous expression of PCP-GFP

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PCP-GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPRIME-CMV-dsRed-recipient

    Plasmid

    #11658

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IVT-MCP-MMLV-RT

    Plasmid

    #207457

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFlag-CMV2-WBP2

    Plasmid

    #27478

    Depositor

    Marius Sudol

    Article

    Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

    Insert

    WW domain binding protein-2 (WBP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Use

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mt TEFM

    Plasmid

    #205058

    Purpose

    TEFM protein expression

    Depositor

    Smita Patel

    Article

    Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

    Insert

    TEFM (TEFM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3TERM_35S

    Plasmid

    #136114

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. UPmCh

    Plasmid

    #161004

    Purpose

    ubiquitous expression of PCP-mCherry

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PCP-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UMsfG

    Plasmid

    #161005

    Purpose

    ubiquitous expression of MCP-sfGFP

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    MCP-sfGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. POLN_pDEST17

    Plasmid

    #64885

    Purpose

    bacterial expression of POLN for purification

    Depositor

    Richard Wood

    Article

    Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

    Insert

    POLN (POLN Human)
    

    Use

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pQFlag-USP11 WT puroR

    Plasmid

    #46747

    Purpose

    Retroviral vector that expresses Flag-tagged wild type human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge