We narrowed to 2,463 results for: T2A

1. attB-puro donor.dna

   Plasmid

   #181923

   Purpose

   attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attB-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-sgAAVS1-2

   Plasmid

   #129727

   Purpose

   Expressing SpCas9 and sgAAVS1-2

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-2
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. attP-puro donor

   Plasmid

   #182139

   Purpose

   attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-mcherry

   Plasmid

   #161974

   Purpose

   Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Cas9-t2a-mcherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN(CMV-copGFP-Puro-SMAR)

   Plasmid

   #80391

   Purpose

   expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Puromycine
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn1-FLEX-CybGam-WPRE

   Plasmid

   #207662

   Purpose

   Control for CybSEP2

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   Gamillus
   

   Use

   AAV

   Mutation

   Histidine 86 and 159 to alanines, inserted gamill…

   Promoter

   hSynapsin

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-APOBEC1-YTH

   Plasmid

   #178949

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTH-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Promoter

   tight TRE

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL_U2AF1_WT_mCherry

   Plasmid

   #84017

   Purpose

   expression of U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

    Plasmid

    #197687

    Purpose

    plasmid for expressing the DNMT3bCD

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-PS Intein

    Plasmid

    #242020

    Purpose

    AAV version of PS Intein palsmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein
    

    Use

    Adenoviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-Cas9

    Plasmid

    #89995

    Purpose

    Expresses high levels of WT Cas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

    Insert

    hCas9
    

    Tags

    3xFlag, SV40 NLS, and T2A peptide

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. eGFP Y93H CC editing reporter

    Plasmid

    #198885

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGC)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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