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Showing: 1801 - 1820 of 4301 results

  3. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  5. P9_TEVs_cLuc

    Plasmid
    #119303
    Purpose
    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc connected to P9 coil via TEVs cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119303-plasmid-map-sequence-id-231805

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  6. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid
    #124660
    Purpose
    reporter protein which emits bright blue fluorescence
    Depositor
    Yutaka Kuroda
    Article
    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1
    Insert
    Gaussia luciferase-C9D-TGinsert
    Use
    Tags
    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…
    Expression
    Bacterial
    Mutation
    changed glutamic acid 100 to alanine, and glycine…
    Promoter
    T7 promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    124660-plasmid-map-sequence-id-255641

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  11. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119299-plasmid-map-sequence-id-231827

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  15. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

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  16. psicheck-2-LPPRC-mod

    Plasmid
    #48168
    Purpose
    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element
    Depositor
    Vamsi Mootha
    Article
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Insert
    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    A/T mutation in the ATG of the uORF
    Promoter
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1820 of 4301 results