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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8

Plasmid

#99354

Purpose

Bacterial expression of fission yeast (S. pombe) Tropomyosin, cdc8

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKate2-T2A-mTurquoise2

Plasmid

#118426

Purpose

Produces equimolar expression of mKate2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mKate2-T2A-mTq2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSEYC68-HA-TOR2 (pAS21)

Plasmid

#39207

Depositor

Michael Hall

Article

Kunz et al J Biol Chem. 2000 Nov 24;275(47):37011-2

Insert

TOR2 (TOR2 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

GAL

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-YPT31

Plasmid

#105263

Purpose

Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt31 (YPT31 )

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pVP64

Plasmid

#22287

Depositor

Louise Prakash

Article

Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

Insert

REV1 (REV1 Budding Yeast)

Tags

4xHA

Expression

Yeast

Available Since

April 22, 2010

Availability

Academic Institutions and Nonprofits only
pCfB8383

Plasmid

#126917

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mScarlet-T2A-mTurquoise2

Plasmid

#118431

Purpose

Produces equimolar expression of mScarlet and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mScarlet-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mVenus-T2A-mTurquoise2

Plasmid

#118434

Purpose

Produces equimolar expression of mVenus and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mVenus-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ221

Plasmid

#132952

Purpose

crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SNX4-iGFPx3

Plasmid

#105269

Purpose

Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snx4 portion (SNX4 Budding Yeast)

Tags

iGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCH001

Plasmid

#47953

Purpose

tetO-GFP reporter casette

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

GFP

Use

Tet inducible expression

Expression

Yeast

Promoter

T3

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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YIplac211-KEX2-iGFPx6

Plasmid

#105264

Purpose

Plasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Kex2 portion (KEX2 )

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pVEG17*

Plasmid

#111856

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

Depositor

Nikhil Nair

Article

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL1p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL1p

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tdTomato-T2A-mTurquoise2

Plasmid

#118439

Purpose

Produces equimolar expression of tdTomato and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tdTomato-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pMV13

Plasmid

#128864

Purpose

Position 1 module- CEN (Centromeric)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CEN/ARS

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJJH1242

Plasmid

#36909

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mRuby2-T2A-mTurquoise2

Plasmid

#118429

Purpose

Produces equimolar expression of mRuby2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mRuby2-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCH043

Plasmid

#47949

Purpose

rtTA under the control of GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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