We narrowed to 1,254 results for: sumo

1. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. WDV-Echistatin

   Plasmid

   #226985

   Purpose

   Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Inserts

   WDV (repA )
   Echistatin
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF-rM1PYK

   Plasmid

   #220232

   Purpose

   Inducible expression of rabbit muscle pyruvate kinase isoform 1

   Depositor

   Aron Fenton, Liskin Swint-Kruse

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Rabbit muscle pyruvate kinase (PKM )
   

   Use

   Tags

   6xHis-MBP-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHTSHP-Smt-IIShhN

   Plasmid

   #121135

   Purpose

   Expresses N-terminal SUMO tagged II-ShhN in bacteria

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Shh (Shh Mouse)
   

   Use

   Tags

   HIS, HRV3C site, MBP, and Smt tag

   Expression

   Bacterial

   Mutation

   Amino acid sequence from 26-195, with two extra i…

   Promoter

   tac

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 huPQBP1

   Plasmid

   #217449

   Purpose

   Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   PQBP1 (PQBP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 IST1 (322-366)

   Plasmid

   #193038

   Purpose

   Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 322-366

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 IST1 L328D, L355A (1-366)

   Plasmid

   #193040

   Purpose

   Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, L355A

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CAPN7 L61D (1-165)

   Plasmid

   #193033

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L61D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 CAPN7 V18D,F98D (1-165)

   Plasmid

   #193034

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   V18D, F98D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 IST1, residues 316-366, C-Cys

    Plasmid

    #191234

    Purpose

    Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 316-366, contains nonnative C-terminus f…

    Promoter

    T7

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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