We narrowed to 8,358 results for: BLI;

1. pCAG-eCas9-GFP-U6-gRNA

   Plasmid

   #79145

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

   Depositor

   Jizhong Zou

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMB52/14-3-3_1_247

   Plasmid

   #40541

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   YWHAG (YWHAG Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1-247

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pML104-KanMx4

   Plasmid

   #83476

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   KanMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL31

   Plasmid

   #227428

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

   Depositor

   Pierre Santucci

   Insert

   mWasabi
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL32

   Plasmid

   #227429

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

   Depositor

   Pierre Santucci

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

   Plasmid

   #203349

   Purpose

   Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   mGreenLantern
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. IRES-creERT2-FNF

   Plasmid

   #188230

   Purpose

   EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette

   Depositor

   Mario Capecchi

   Insert

   IRES-creERT2-FNF
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG-6a-Ataxin3Q22

    Plasmid

    #22126

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

11. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mArg1-Flag

    Plasmid

    #34574

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(+) Drp1

    Plasmid

    #34706

    Depositor

    David Chan

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTipi2.1-AZU_SP

    Plasmid

    #229144

    Purpose

    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. SARS-CoV Nucleocapsid

    Plasmid

    #154269

    Purpose

    Expresses myc-tagged nucleocapsid (N) from SARS-CoV (1) in mammalian cells

    Depositor

    Peter Klein

    Insert

    SARS-CoV Nucleocapsid
    

    Use

    Tags

    5X myc epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCMV14_Fibrillarin

    Plasmid

    #73061

    Purpose

    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1 CCNE1

    Plasmid

    #164144

    Purpose

    Doxycycline inducible expression of Cyclin E1

    Depositor

    Richard Possemato

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-phSyn-flex-SypEGFP

    Plasmid

    #153203

    Purpose

    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Lynette Lim

    Insert

    synaptophysin-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat synapsin

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGR596

    Plasmid

    #213787

    Purpose

    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    See manuscript

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pScarlessHD-sfGFP-DsRed

    Plasmid

    #80811

    Purpose

    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    sfGFP-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-hPAX7 (#348)

    Plasmid

    #78337

    Purpose

    Overexpression of human PAX7 ORF with a N-terminal FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX7 (PAX7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

23. GATA3-pCDNA3.1

    Plasmid

    #115271

    Purpose

    Expresses human GATA3 in mammalian cells

    Depositor

    Arne S. Schaefer

    Insert

    GATA3 (GATA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid

    #102996

    Purpose

    Expresses synaptophysin fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Insert

    Synaptophysin-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Minos ITR loxp MCS lox2272

    Plasmid

    #218983

    Purpose

    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site

    Depositor

    Amro Hamdoun

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSK-mPar1

    Plasmid

    #53227

    Purpose

    bacterial expression of mouse Par1

    Depositor

    Shaun Coughlin

    Insert

    Par1 (F2r Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBABEpuro-CRAF

    Plasmid

    #51124

    Purpose

    mammalian expression of wt CRAF

    Depositor

    Matthew Meyerson

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-TRE-TdTomato

    Plasmid

    #110338

    Purpose

    TdTomato reporter vector of iTango and Cal-Light system for AAV production

    Depositor

    Hyungbae Kwon

    Insert

    tetO-TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-mOct4 Full length(FL)

    Plasmid

    #206394

    Purpose

    Expresses Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EF1α-mNeonGreen-Z1

    Plasmid

    #135613

    Purpose

    mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    mNeonGreen
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-HTLV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pScarlessHD-3xFLAG-DsRed

    Plasmid

    #80820

    Purpose

    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    3xFLAG-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGR618

    Plasmid

    #213786

    Purpose

    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

    Depositor

    Chang Liu

    Insert

    BB-Tv TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZDonor rosa26 LSL-mMALAT1

    Plasmid

    #140691

    Purpose

    generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region

    Depositor

    Jianjun Zhao

    Insert

    mouse MALAT1 (Malat1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. K44A HA-dynamin 1 pcDNA3.1

    Plasmid

    #34683

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SETD7

    Plasmid

    #40746

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD7 (SETD7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. His-RIAM

    Plasmid

    #32803

    Depositor

    Vicki Boussiotis

    Insert

    RIAM (APBB1IP Human)
    

    Use

    Tags

    His and XPress

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge