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pDY0739 pCAG-NLS-SacINT

Plasmid

#179114

Purpose

SacINT expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCAG-NLS-SacINT

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Eomes-3xT7

Plasmid

#200888

Purpose

Inducible lentiviral expression of Eomes

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Eomes (Eomes Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDY0312 PhiBT Integrase

Plasmid

#179111

Purpose

PhiBT Integrase expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

PhiBT Integrase

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-R2505P

Plasmid

#69015

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-R2505P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Arginine 2505 to Proline

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239030

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse VGAT (Slc32a1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
M3-M14-dCas9

Plasmid

#134779

Purpose

Expression of fusion protein M3-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

Plasmid

#210501

Purpose

expresses LAUNCHER component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

Plasmid

#175554

Purpose

Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

NANOG

Use

Mouse Targeting

Tags

FKBPV-HA2-P2A-eGFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-I2500F

Plasmid

#69014

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-I2500F (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Isoleucine 2500 to Phenylalanine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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