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Plasmid#213308PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCA2 (HCAR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-6xTRE-FLEX-hM4Di.mCherry
Plasmid#169415PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expressionDepositorInserthM4Di.mCherry
UseAAVTagsmCherryExpressionMutationhM4Di is Gi-coupled DREADD.Derived from human mus…PromoterMinimal TATAAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21-P369S-MEFV
Plasmid#134712PurposeLentiviral expression of human MEFV P369S after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.P369SPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M680I-MEFV
Plasmid#134711PurposeLentiviral expression of human MEFV M680I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M680IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694I-MEFV
Plasmid#134710PurposeLentiviral expression of human MEFV M694I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-S208C/M694V-MEFV
Plasmid#134709PurposeLentiviral expression of human MEFV S208C/M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.S208C/M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694V-MEFV
Plasmid#134703PurposeLentiviral expression of human MEFV M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(-KTS)
Plasmid#220565PurposeLentiviral expression of human EWSR1::WT1(-KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(+KTS)
Plasmid#220566PurposeLentiviral expression of human EWSR1::WT1(+KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP
Plasmid#176278PurposeViral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertKir2.1-P2A-EGFP (Kcnj2 Synthetic, Mouse)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only