We narrowed to 23,976 results for: nes

1. Thy1-Brainbow-1.0 H

   Plasmid

   #18724

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   tdimer2 ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAIO3

   Plasmid

   #137844

   Purpose

   Cas9 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCJ163

   Plasmid

   #175056

   Purpose

   rox flanked gentamycin resistance cassette integrates into A2542 integration site

   Depositor

   David Nielsen

   Article

   Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

   Insert

   rox-mR-rox
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS303-pGAL-HA-SrtAstrep (pKS78)

   Plasmid

   #50029

   Purpose

   Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   HA-SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   pGAL

   Available since

   Jan. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX ALIX-GFP S718/721/E

   Plasmid

   #138584

   Purpose

   Expresses fluorescent ALIX S718/721E in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Mutation

   changed serines 718 and 721 to glutamic acids

   Promoter

   TREtight

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ164

   Plasmid

   #175057

   Purpose

   rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL

   Depositor

   David Nielsen

   Article

   Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

   Insert

   DRE
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   rbcLXS

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Venus-79aa-ActA-pEGFP-C1

   Plasmid

   #166763

   Purpose

   To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   ActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

   Plasmid

   #132927

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Insert

   humanized SpCas9
   

   Use

   CRISPR and Synthetic Biology; Pmagic gateway entr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDCAF-B

   Plasmid

   #113647

   Purpose

   Demethylates methylxanthines.

   Depositor

   Jeffrey Barrick

   Article

   Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

   Insert

   ndmB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDCAF-C

    Plasmid

    #113648

    Purpose

    Demethylates methylxanthines.

    Depositor

    Jeffrey Barrick

    Article

    Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

    Insert

    ndmC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pDCAF-AB

    Plasmid

    #113649

    Purpose

    Demethylates methylxanthines.

    Depositor

    Jeffrey Barrick

    Article

    Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

    Insert

    ndmA and ndmB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDCAF-AC

    Plasmid

    #113650

    Purpose

    Demethylates methylxanthines.

    Depositor

    Jeffrey Barrick

    Article

    Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

    Insert

    ndmA and ndmC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDCAF-BC

    Plasmid

    #113651

    Purpose

    Demethylates methylxanthines.

    Depositor

    Jeffrey Barrick

    Article

    Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

    Insert

    ndmB and ndmC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDCAF-A

    Plasmid

    #113646

    Purpose

    Demethylates methylxanthines.

    Depositor

    Jeffrey Barrick

    Article

    Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

    Insert

    ndmA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMin1

    Plasmid

    #102959

    Purpose

    Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions

    Depositor

    Rainer Haas

    Article

    Kahrs et al Gene. 1995 Dec 29;167(1-2):53-7.

    Type

    Empty backbone

    Use

    Mini-transposon system suitable for sequencing, s…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MP-P11

    Plasmid

    #69670

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 mutSdN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ilux pGEX(-)

    Plasmid

    #107879

    Purpose

    Bacterial expression of ilux for bioluminescence imaging

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

    Insert

    ilux
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJPC210

    Plasmid

    #234532

    Purpose

    Int13 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxOFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV mouse sidekick2

    Plasmid

    #18735

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

    Insert

    Sidekick-2 (Sdk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pBAD-luxKate

    Plasmid

    #229946

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxKate
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD-luxKOk

    Plasmid

    #229944

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxKOk
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBAD-luxGold

    Plasmid

    #229943

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxGold
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV mouse sidekick1

    Plasmid

    #18734

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

    Insert

    Sidekick-1 (Sdk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-Plk1(829)

    Plasmid

    #39845

    Depositor

    Jonathon Pines

    Article

    Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3-luxOFP

    Plasmid

    #229950

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxOFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-luxNeon

    Plasmid

    #229947

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxNeon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pccGFPGpA

    Plasmid

    #73651

    Purpose

    Fluorescent reporter for transmembrane protein dimerization positive control.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Insert

    glycophorin A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge