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pMOD_C2519

Plasmid

#91088

Purpose

Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

OsU3

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)

Plasmid

#83465

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_B2000

Plasmid

#91059

Purpose

Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Promoter

none (begins with P2A to be fused to TALEN_1)

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2403

Plasmid

#91071

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJK597

Plasmid

#71121

Purpose

Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDL649

Plasmid

#231164

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlHAIRLESS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDL518

Plasmid

#231160

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEE771

Plasmid

#231159

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEE485

Plasmid

#231158

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-1pro

Plasmid

#231149

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbSTM-5'UTR

Plasmid

#231148

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-2pro and NbATML1-1pro

Plasmid

#231155

Purpose

T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL655

Plasmid

#231165

Purpose

T-DNA encoding gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

gRNA targeting SlPDS

Expression

Plant

Promoter

AtU6

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL562

Plasmid

#231162

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlCHL1

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEE834

Plasmid

#231168

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-1125

Plasmid

#217999

Purpose

Start-methionine

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Start-Met

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA333

Plasmid

#216067

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; PuroR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA371

Plasmid

#215989

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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