We narrowed to 5,366 results for: pcDNA3.1+

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pCDNA3.1-Flag-H2A K13-15R

Plasmid

#63563

Purpose

Expression of human H2A with K13R and K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 13 and Lysine 15 are now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

Plasmid

#75203

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 T239A

Plasmid

#22219

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 CMV mCherry-eGFP BGH

Plasmid

#92280

Purpose

Control FRET construct; tandem mCherry-eGFP fusion

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

mCherry-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

Plasmid

#112157

Purpose

Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

Depositor

Ben Giepmans

Article

de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

Insert

anti-GFP FLIPPER-body

Tags

His and Thrombin cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dL5-2XG4S-actin

Plasmid

#73262

Purpose

Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

Insert

KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)

Tags

The FAP and actin are fused with 2 copies of a G4…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

Plasmid

#171432

Purpose

Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-hH3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) L533E V5 His

Plasmid

#119288

Purpose

CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Tags

V5 His

Expression

Mammalian

Mutation

L533E

Promoter

T7

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/FGFR2-S252W(Apert) myc

Plasmid

#33249

Depositor

Claudio Basilico, Alka Mansukhani

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Tags

His and Myc

Expression

Mammalian

Mutation

Mutation S252W in FGFR2 found in Apert syndrome. …

Promoter

CMV

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

Plasmid

#114111

Purpose

fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

Insert

Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 LIC 6A HIS-TEV-DPP9S

Plasmid

#166791

Purpose

Mammalian cell expression vector for His-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

Plasmid

#193997

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181_NLS

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181_NLS

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

Plasmid

#127314

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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