We narrowed to 7,531 results for: ski

1. pMXS-IRES-Blast POLD1 ISCmut

   Plasmid

   #160806

   Purpose

   Express ISC mutant POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 ISCmut (POLD1 Human)
   

   Use

   Retroviral

   Mutation

   Codon optimized C-terminal 230 amino acids, C1058…

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His6-SUMO-Lyn-SH2-AviTag

   Plasmid

   #214207

   Purpose

   Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

   Insert

   Lyn A (LYN Human)
   

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

   Plasmid

   #214231

   Purpose

   Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.

   Depositor

   Neel Shah

   Article

   Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

   Insert

   Grb2 SH2 domain (GRB2 Human)
   

   Tags

   6xHis, AviTag, and mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-His6-SUMO-Lck-SH2-AviTag

   Plasmid

   #214203

   Purpose

   Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

   Insert

   Lck kinase SH2 domain (LCK Human)
   

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His6-SUMO-Blk-SH2-AviTag

   Plasmid

   #214204

   Purpose

   Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

   Insert

   Blk kinase SH2 domain (BLK Human)
   

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-His6-SUMO-Hck-SH2-AviTag

   Plasmid

   #214206

   Purpose

   Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

   Insert

   p60-HCK (HCK Human)
   

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PAX3::FOXO1-2A-sfGFP

   Plasmid

   #240098

   Purpose

   human PAX3::FOXO1 in -2A-sfGFP backbone (Plasmid# 74668)

   Depositor

   Genevieve Kendall

   Article

   Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

   Insert

   PAX3::FOXO1 (FOXO1, PAX3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-TAZ-CAMTA1

   Plasmid

   #235681

   Purpose

   Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

   Depositor

   Duojia Pan

   Article

   Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

   Insert

   TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233046

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid

    #233045

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. LENTICRISPR-GPI_sgRNA2

    Plasmid

    #201595

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid

    #201677

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-3g-EEA-2g-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV Cdk2ap1ΔN (MT2B2)-HA

    Plasmid

    #178031

    Purpose

    Retroviral vector for the purpose of overexpression in mammalian cell culture

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    GAG

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-EGFP MASTL E167D (siRNA resistant)

    Plasmid

    #191013

    Purpose

    Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E167D

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-Blast POLA1 ISCmut

    Plasmid

    #160808

    Purpose

    Express ISC mutant POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLA1 ISCmut (POLA1 Human)
    

    Use

    Retroviral

    Mutation

    Codon optimized, C1354S, C1359S, C1377S, C1380S

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid

    #113701

    Purpose

    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid

    #113699

    Purpose

    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSyn.iGluSnFR3.v857.PDGFR

    Plasmid

    #178329

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGG-AH-VRT-A2

    Plasmid

    #163703

    Purpose

    Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)

    Depositor

    Cristobal Uauy

    Article

    Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.

    Inserts

    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
    

    Expression

    Plant

    Promoter

    Rice - Os Actin promoter and Triticum polonicum V…

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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