We narrowed to 1,186 results for: MS2;

1. scFv-GCN4-DNMT3a-DNMT3l

   Plasmid

   #154140

   Purpose

   Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR2.1_HBB_N

   Plasmid

   #87847

   Purpose

   pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

   Depositor

   Marina Kleanthous

   Article

   Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

   Insert

   Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LIC-Z

   Plasmid

   #153543

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LIC-Z-delCry2

   Plasmid

   #153545

   Purpose

   this is the non-clustering version of LIC-Z tagged with mcherry

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   with Cry2 sequence deleted from LIC-Z

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

   Plasmid

   #160458

   Purpose

   Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Inserts

   PAX7 Left/5' Homology Arm (PAX7 Human)
   PAX7 Right/3' Homology Arm (PAX7 Human)
   

   Use

   Insertion of a fluorescent reporter through homol…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LIC-Z-YFP

   Plasmid

   #153544

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Venus YFP

   Expression

   Mammalian

   Mutation

   with mCherry in LIC-Z replaced by YFP

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Mutation

   Promoter

   osk

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE3G-EGFPC-uL10-(Neo)R

   Plasmid

   #158069

   Purpose

   eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

   Depositor

   Adolfo Rivero-Muller

   Article

   Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

   Insert

   RPLP0 (RPLP0 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC39A4

   Plasmid

   #132069

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC39A4 (SLC39A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCW57-Cx46-IRES-GCaMP6s

    Plasmid

    #188238

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJA3 (GJA3 Human)
    

    Use

    Lentiviral

    Tags

    GCaMp6s

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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