We narrowed to 4,212 results for: Pet2

1. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Use

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28 His6-mEGFP-D4

   Plasmid

   #226372

   Purpose

   Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4 (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-SpG-His

   Plasmid

   #179318

   Purpose

   Plasmid for bacterial expression and purification of SpG-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpG
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-T7-6h-GST-α-Synuclein

   Plasmid

   #225224

   Purpose

   Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   GST
   α-Synuclein
   

   Use

   Tags

   6xHis Tag and TEV Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-MH6-EsCas13d

   Plasmid

   #108303

   Purpose

   Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   EsCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LOV-Turbo-His6_pET21a

   Plasmid

   #199668

   Purpose

   expresses LOV-Turbo in bacteria

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-GBP-MTn

    Plasmid

    #154363

    Purpose

    Bacterial expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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