Purpose
              
              Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
             
          
          
          
            
          
        
          
  
  
    
      
        Insert
      
      
        
          IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
        
      
    
   
  
  
  
  
    
  
  
    
      
        Mutation
      
      Y32G L65E F108W Q109M D158S R162K
     
  
  
    
      
        Promoter
      
      lpp (constitutive)
     
  
         
          
            
              
                Available Since
              
              
                March 2, 2017
              
             
          
          
            
              Availability
            
            
              
                Academic Institutions and Nonprofits only