We narrowed to 6,121 results for: cas9 expression plasmid
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Plasmid#169441PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Stuffer
Plasmid#106248PurposeDestination vector for U6::gRNA expression cassetteDepositorTypeEmpty backboneUseAAVAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8381
Plasmid#126915PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1DepositorInsertXdCrtI and tHMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeurog2-Luciferase reporter
Plasmid#64126PurposePhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.DepositorInsertNeurog2 promoter
UseLuciferaseTagsluciferaseExpressionMammalianPromoterNeurog2Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAS_gpyrG1
Plasmid#90276Purpose(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9DepositorInsertgRNA (pyrg1)
UseA. nigerExpressionBacterialAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLS234
Plasmid#73682PurposeSapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP608-1
Plasmid#99484PurposeH2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTRDepositorInsertH2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD401
Plasmid#91828PurposePmyo-2::GFP FP-slot donor for the SapTrap cloning systemDepositorInsertPmyo-2::GFP
UseCRISPRExpressionWormMutationNucleotide 489 of the myo-2 promoter was changed …Available SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-TREX2-N
Plasmid#244025PurposeGateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoterDepositorInsertCas12a
UseCRISPRTagsTREX2Available SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-AtEXO1B-N
Plasmid#244024PurposeGateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoterDepositorInsertCas12a
UseCRISPRTagsAtEXO1BAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWR95(URA3)
Plasmid#203917PurposeBLINCAR plasmid for gene deletion, URA3 targetDepositorInsertsURA3 upstream homology
URA3 downstream homology
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR95
Plasmid#203916PurposeBLINCAR plasmid for gene deletion, no genomic targetDepositorInsertmodified MCS
ExpressionYeastAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS_gpyrG2
Plasmid#90277Purpose(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9DepositorInsertgRNA (pyrg2)
UseA. nigerExpressionBacterialAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP1071-2
Plasmid#99502Purpose3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEVDepositorInsert3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVV205
Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23105Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIK127
Plasmid#65631PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.DepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR
ExpressionWormAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only