Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,632 results for: t7

Showing: 1881 - 1900 of 10632 results

  1. pcDNA3.1_C-6His-Stx17

    Plasmid
    #112259
    Purpose
    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)
    Depositor
    Madhulika Tripathi
    Insert
    syntaxin 17 (Stx17 Mouse)
    Tags
    6His Tag
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112259-plasmid-map-sequence-id-217085

    Enlarge

  2. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

    Enlarge

  3. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid
    #169377
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP2
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169377-plasmid-map-sequence-id-335058

    Enlarge

  4. pmCellFree_KA1_SARS-COV-2_NSP3

    Plasmid
    #169378
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP3
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169378-plasmid-map-sequence-id-335064

    Enlarge

  5. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

    Enlarge

  6. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

    Plasmid
    #46179
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Tags
    ECFP, EYFP, and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (Vt, tail domain)
    Promoter
    T7
    Available Since
    July 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    46179-plasmid-map-sequence-id-198213

    Enlarge

  7. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

    Enlarge

  8. LHP2691 - pET30-Ub-K63A

    Plasmid
    #32179
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K63A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. LHP2690 - pET30-Ub-K48A

    Plasmid
    #32178
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K48A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pET3a-His-DJ1

    Plasmid
    #51488
    Purpose
    Inducible expression of human His-DJ1 in E.coli
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    DJ1 (PARK7 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

    Enlarge

  12. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. SLX4

    Plasmid
    #73242
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLX4 (SLX4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73242-plasmid-map-sequence-id-136207

    Enlarge

  14. pmCellFree_KA1_SARS-COV-2_ORF9b

    Plasmid
    #169396
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169396-plasmid-map-sequence-id-335426

    Enlarge

  15. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

    Enlarge

  16. pmCellFree_KA1_SARS-COV-2_NSP9

    Plasmid
    #169383
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP9
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169383-plasmid-map-sequence-id-336869

    Enlarge

  17. TIF1A

    Plasmid
    #74647
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TIF1A (TRIM24 Human)
    Tags
    His-6-SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

    Enlarge

  19. pmCellFree_KA1_SARS-COV-2_NSP12

    Plasmid
    #169385
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP12
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169385-plasmid-map-sequence-id-336500

    Enlarge

  20. SHC1

    Plasmid
    #73249
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SHC1 (SHC1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73249-plasmid-map-sequence-id-136213

    Enlarge

Showing: 1881 - 1900 of 10632 results