165,688 results

1. pLyGo-Ec-8

   Plasmid

   #163136

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P(rhaB)

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHiUGE HA-epitope donor ORF0

   Plasmid

   #200371

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-PKCA D463N

   Plasmid

   #195203

   Purpose

   Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

   Depositor

   Peter Parker, David Bryant

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Expression

   Mammalian

   Mutation

   D463N

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-VSVG

   Plasmid

   #35616

   Depositor

   Arthur Nienhuis, Patrick Salmon

   Insert

   VSV G protein
   

   Expression

   Mammalian

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTECH-chAcK3RS(IPYE)

   Plasmid

   #104069

   Purpose

   Expression plasmid encoding chAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chAcK3RS(IPYE); PproK pyl
   

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

   Plasmid

   #188375

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWM_12x601_15bpLinker

   Plasmid

   #157786

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_15bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Parkin

    Plasmid

    #17613

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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