We narrowed to 2,458 results for: GEM

1. pLenti-CaMKIIa-ChETA-EYFP

   Plasmid

   #26967

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T

   Promoter

   CaMKIIa

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. p006-Banana-Late

   Plasmid

   #112251

   Purpose

   ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   ATF1 under osmY promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   osmY

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/VChR1-EYFP

   Plasmid

   #20955

   Purpose

   Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

   Insert

   volvox channelrhodopsin-1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGCG26

   Plasmid

   #82371

   Purpose

   YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pALM179

   Plasmid

   #82023

   Purpose

   an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

   Depositor

   Brian Pfleger

   Article

   Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas49

   Plasmid

   #82391

   Purpose

   rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   rbcL-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PccmK2

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p005kanRFP

   Plasmid

   #58533

   Purpose

   RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5 between two lac operators

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus Cam KII alpha mutant T286D

   Plasmid

   #29429

   Depositor

   Steven Vogel

   Article

   Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

   Insert

   Venus CAM KII Alpha
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T286D mutation in CamKIIa sequence

   Promoter

   Available since

   July 5, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pSB3C5-proD-B0032-E0051

   Plasmid

   #107241

   Purpose

   Expresses lacZa-GFP fusion from constitutive proD promoter

   Depositor

   Robert Sauer, Joseph Davis

   Article

   Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

   Insert

   ProA-RBS(B0032)-Gemini (E0051)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   proD

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

    Plasmid

    #20293

    Purpose

    Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Alanine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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