2,374 results
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Plasmid#236396PurposeExpresses FLAG-Rgs14 in mammalian cellsDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
TagsFLAGExpressionMammalianAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-LOV2-OMP25
Plasmid#233346PurposeStable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.DepositorInsertsUseRetroviralTagsEGFPExpressionMammalianMutationN-terminus is deleted and N538EAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_R-PTEN_4A
Plasmid#227435PurposeExpression of the R-PTEN sensor with a 4A mutationDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG_G-PTEN
Plasmid#227436PurposeExpresses the G-PTEN sensor under a CAG promoterDepositorInsertG-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)
TagsmEGFP, and sREAChExpressionMammalianMutationR14GPromoterCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-CAG_G-PTEN
Plasmid#227438PurposeAn AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.DepositorInsertG-PTEN sensor (Pten Rat)
UseAAVTagsCD-sREACh and mEGFPExpressionMammalianMutationR14GPromoterpCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_G-PTEN_4A
Plasmid#227434PurposeExpresses the G-PTEN sensor with a 4A mutationDepositorInsertG-PTEN 4A (Pten Rat)
TagsmEGFP and sREAChExpressionMammalianMutation4A mutation.PromoterCMVAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP-TEV-Rat BCKDK-His
Plasmid#232119PurposeFor bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.DepositorInsertBCKDK (Bckdk Rat)
Tags6 x His, Maltose-Binding Protein (MBP), and Tev S…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_5HFD
Plasmid#234189PurposeHeterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1EYH
Plasmid#234159PurposeHeterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
Tags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
Tags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
Tags6xHis, thrombinExpressionBacterialAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
Tags6xHis, thrombinExpressionBacterialAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold2s-Lysosome-N-20
Plasmid#231770PurposeVisualization of lysosome in mammalian cells using mGold2sDepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-EGFP
Plasmid#209324PurposeAAV packaging vector containing a APOBEC1 expression, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1-HA
UseAAVTagsHAAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-APOBEC1
Plasmid#229537PurposepMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
dnCas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-APOBEC1
Plasmid#229536PurposepMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
Cas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only