2,294 results

1. An R57A, R59A CAAX pMT2

   Plasmid

   #206119

   Purpose

   Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1a (Cacna1a Rat)
   

   Expression

   Mammalian

   Mutation

   R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. cacna2d1 R241A pMT2

   Plasmid

   #206110

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Mutation

   R241A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. cacna2d1 R241A HA pMT2

   Plasmid

   #206111

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Mutation

   R241A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. An HA CAAX pMT2

   Plasmid

   #206116

   Purpose

   expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1a (Cacna1a Rat)
   

   Expression

   Mammalian

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cacna2d1 BBS pcDNA3

   Plasmid

   #206106

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

   Depositor

   Annette Dolphin

   Article

   Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Cav2.1 R57A, R59A pMT2

   Plasmid

   #206112

   Purpose

   expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

   Insert

   cacna1a (Cacna1a Rat)
   

   Expression

   Mammalian

   Mutation

   R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. rUBR5_2383-2442_pGEX6P1

   Plasmid

   #209791

   Purpose

   Bacterial expression of UBR5 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

   Insert

   Mlle domain of UBR5 (Ubr5 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2383-2442 only

   Promoter

   tac

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETSyt1C2AB(80-421)msGFP

   Plasmid

   #209009

   Purpose

   Recombinant protein expression of truncated syt1 tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETSUMOSyt1C2AB(96-421)

   Plasmid

   #209006

   Purpose

   Recombinant protein expression of truncated syt1 construct

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 96-421 only

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETmsGFPSyt1(80-142)

    Plasmid

    #209012

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis-msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-142 only

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETmsGFPSyt1(80 JuxtaK-142)

    Plasmid

    #209013

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis-msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETSyt1C2AB(143-421)msGFP

    Plasmid

    #209010

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 143-421 only

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETSUMOSyt1C2AB(80 JuxtaK-421)

    Plasmid

    #209008

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETSUMOSyt1C2AB(143-421)

    Plasmid

    #209007

    Purpose

    Recombinant protein expression of truncated syt1 construct

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 143-421 only

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209011

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFSynWSyt1C2AB(143-421)msGFP

    Plasmid

    #209015

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 143-421 only

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. An R57A, R59A HA CAAX pMT2

    Plasmid

    #206126

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. An CAAX pMT2

    Plasmid

    #206118

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMHE-RnCENP-O-GFP

    Plasmid

    #205179

    Purpose

    Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo Rat)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Wild type rat CENP-O

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge