2,226 results

1. pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro

   Plasmid

   #198850

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND1-W86-L14 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro

   Plasmid

   #198858

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND5-W68-L15 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro

   Plasmid

   #198846

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX3-W16-L17 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

   Plasmid

   #198840

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP8-W9-L17 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro

   Plasmid

   #198854

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND3-Q26-L18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro

   Plasmid

   #198838

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP6-W68-L16 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro

   Plasmid

   #198856

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND4-W24-L18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

   Plasmid

   #198844

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-L18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

   Plasmid

   #198852

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND2-W30-L16 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

    Plasmid

    #198848

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    CYTB-W77-L15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

    Plasmid

    #198842

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    COX1-W25-L15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

    Plasmid

    #192339

    Purpose

    Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

    Plasmid

    #192317

    Purpose

    Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe NGF-Erbb2-2xFKBP-HA hygro

    Plasmid

    #192291

    Purpose

    Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

    Plasmid

    #198860

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND6-W82-L15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

    Plasmid

    #194016

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.2)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmb and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI-neo-μ3A-(HA)3

    Plasmid

    #198179

    Purpose

    Expression of HA-tagged AP-3 μ3A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

    Insert

    AP-3 μ3A
    

    Use

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent substitution in codon 225

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B52_puro_gRNA_3418711_KCNB2

    Plasmid

    #197558

    Purpose

    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

    Depositor

    Darius Bägli

    Insert

    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    

    Use

    Grna with puromycin selection

    Tags

    puromycin resistance cassette under CMV promotion

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RCav3.1/pcDNA3-HE3

    Plasmid

    #197324

    Purpose

    Plasmid expressing an antigen targeting the Cav3.1 calcium channel subunit

    Depositor

    Edward Perez-Reyes

    Insert

    Cav3.1 (Cacna1g Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GIT1-Flag/pBK

    Plasmid

    #197313

    Purpose

    Plasmid expressing an antigen targeting GIT1

    Depositor

    Eunjoon Kim

    Insert

    GIT1 (Git1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge