We narrowed to 5,531 results for: PEP
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Plasmid#229806PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment siteDepositorInsertSP-mScarlet3-GPI
UseIn vitro transcriptionMutationT7 promoterAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC025 (∆CBBp)
Plasmid#226203PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).DepositorInsertHomology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
UseSynthetic BiologyMutation∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpApAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJRoC30_Th3.1
Plasmid#188065PurposeFuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)DepositorInsertFuncLib-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 PROSS + 4 FuncLib mutations, described in publ…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3.7
Plasmid#188066PurposeFuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)DepositorInsertFuncLib-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 PROSS + 3 FuncLib mutations, described in publ…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3.10
Plasmid#188067PurposeFuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)DepositorInsertFuncLib-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 PROSS + 3 FuncLib mutations, described in publ…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
Tags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-839
Plasmid#217995PurposesAP1 protein secretion signalDepositorInsertSecretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
OXGR1-DuET
Plasmid#213363PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
OXER1-DuET
Plasmid#213362PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX3-DuET
Plasmid#213346PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRE-DuET
Plasmid#213341PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRF-DuET
Plasmid#213342PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR1E-DuET
Plasmid#213319PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCA1-DuET
Plasmid#213307PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCA3-DuET
Plasmid#213309PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR62-DuET
Plasmid#213288PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR32-DuET
Plasmid#213280PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR4-DuET
Plasmid#213283PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR31-DuET
Plasmid#213279PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR148-DuET
Plasmid#213266PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD4-DuET
Plasmid#213230PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR8-DuET
Plasmid#213383PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR9-DuET
Plasmid#213384PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGDR-DuET
Plasmid#213369PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CSII-TK-MAIP1
Plasmid#203599PurposeLentiviral vector to express MAIP1 in mammalian cellsDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLentiviralExpressionMammalianMutationWTPromoterTKAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1
Plasmid#203603PurposeReporter plasmid carrying MAIP1 3'UTRDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseExpressionMammalianPromoterSV40Available SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB4
Plasmid#200983PurposeMammalian expression plasmid for myc-tagged EphB4DepositorInsertEphB4 (EPHB4 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2157
Plasmid#143572PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Usp5
Plasmid#174873PurposeCRISPR vector for generating Usp5 STREAMING-tag KI cellDepositorInsertsgRNA for mouse Usp5 (Usp5 Synthetic)
UseCRISPRAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP10 N67Q N131Q D4G (S2-2)
Plasmid#184622Purposefor transfection into S2 cells and expression of BMP10 proteinDepositorInsertBMP10 (BMP10 Human)
Tags8x His - streptavidin binding peptideExpressionInsectMutationN67Q, N131Q, ARIRR(312-316) replaced with GAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)
Plasmid#155308PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variantDepositorInsertPfAMA1
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationK546M/I547P/I548Y/I549YPromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(M1423R/P1424V)
Plasmid#155309PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationM1423R/P1424V; insert codes for EBA175 residues 1…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424V/Y1425W)
Plasmid#155310PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424V/Y1425W; insert codes for EBA175 residues 1…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424V/Y1426H)
Plasmid#155311PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424V/Y1426H; insert codes for EBA175 residues 1…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(Y1426S)
Plasmid#155035PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationY1426S; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424C)
Plasmid#154978PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424C variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424C; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424M)
Plasmid#154979PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424M variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424M; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424V)
Plasmid#154988PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424V; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424L)
Plasmid#154989PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424L variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424L; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424F)
Plasmid#154990PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424F variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424F; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424Y)
Plasmid#154991PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Y variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424Y; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424W)
Plasmid#154992PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424W variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424W; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424T)
Plasmid#154993PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424T variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424T; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424S)
Plasmid#154994PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424S; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424N)
Plasmid#154995PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424N variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424N; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424Q)
Plasmid#154996PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424Q; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424R)
Plasmid#154997PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424R; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424K)
Plasmid#154998PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424K variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424K; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424H)
Plasmid#154999PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424H; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only