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pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNAOX-twk7

Plasmid

#16106

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Voltage-gated K+ channel (twk-7 Nematode)

Use

Mammalian expression & oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pJJH1308

Plasmid

#36911

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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EML8543_pEML41003

Plasmid

#237864

Purpose

A suicide vector for Agrobacterium with kanamycin resistance.

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYL116

Plasmid

#239967

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Suz12

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYL117

Plasmid

#239968

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Ezh2

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1053

Plasmid

#209543

Purpose

BTK Type 3-4 fluorescent protein cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Type 3-4 E2-Crimson cassette

Use

Synthetic Biology

Promoter

CP25

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 β-Globin

Plasmid

#194437

Purpose

In Situ hybridization probes

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

β-globin

Use

In situ hybridization

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAB228i16

Plasmid

#209738

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i24

Plasmid

#209739

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228r3

Plasmid

#209741

Purpose

ColA BBa_B0014 tetAp SD8 Lum1PylRS-[PylHRS*] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

Mlum1RS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p

Plasmid

#209742

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p4

Plasmid

#209743

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[AF] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Article

Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194726

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-MAFB-sg

Plasmid

#194720

Purpose

px458 with guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

Cas9 (MAFB Streptococcus pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-5.1

Plasmid

#190589

Purpose

Lentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells

Depositor

Jakob Reiser

Article

Bialkowska et al BMC Genomics. 2005 Sep 4;6:113. doi: 10.

Inserts

NeoR transgene
artificial exon encoding BSD and EGFP

Use

Lentiviral

Promoter

Cellular and SV40

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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