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We narrowed to 13,309 results for: sequence

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Showing: 9681 - 9690 of 13309 results

  1. pNOC_hfnCas12a_NR1_NR2_LPT1

    Plasmid
    #176253
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176253-plasmid-map-sequence-id-347028

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  2. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  3. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

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  4. pCEP4-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113948
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    CMV
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113948-plasmid-map-sequence-id-215162

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  5. pCEP4-BG505.SOSIP-QES.i03.c01-8his

    Plasmid
    #111846
    Purpose
    Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 Env (BG505 SOSIP.664)
    Tags
    8his purification tag and CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; has SOSIP mutatio…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111846-plasmid-map-sequence-id-210258

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  6. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  7. pAAV hSyn1 CaRhGC wt T2A tDimer

    Plasmid
    #101720
    Purpose
    humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m
    Depositor
    Thomas Oertner
    Article
    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:
    Inserts
    CatRhGC
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn1 and ribosomal skip sequence T2A
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    101720-plasmid-map-sequence-id-201303

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  8. p426_Cas9_gRNA-ARS416d

    Plasmid
    #87386
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS416d sequence TAGTGCACTTACCCCACGTT in yeast chromosome 4.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS416d
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87386-plasmid-map-sequence-id-193428

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Showing: 9681 - 9690 of 13309 results