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pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10

Plasmid

#243740

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-EASY

Plasmid

#241237

Purpose

CRISPR-Cas9 genome editing

Depositor

Tamara O'Connor

Article

Hossain et al PLoS One. 2024 Mar 14;19(3):e0299513. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA_ASX

Plasmid

#98888

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Article

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

CaMV35S (enhanced)

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA_crE6

Plasmid

#172928

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

Depositor

Arnold Boersma

Article

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorE6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_MOKV_G

Plasmid

#207294

Purpose

Mammalian expression of Mokola virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MOKV_G (G Mokola virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIEx4-Cre

Plasmid

#198280

Purpose

Expresses Cre in insect cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pIEx4-Cre

Expression

Insect

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pHCMV_LASV_GPC

Plasmid

#207287

Purpose

Mammalian expression of Lassa virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LASV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_VSBV_G

Plasmid

#207312

Purpose

Mammalian expression of Variegated Squirrel Bornavirus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSBV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-Rab5

Plasmid

#51612

Purpose

Recruiter to Rab5-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab5

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-N

Plasmid

#58790

Purpose

Express LucN alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 1-398

Tags

FLAG

Expression

Mammalian

Mutation

T576C,T591C to destroy MfeI and EcoRI sites

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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EpAvs1

Plasmid

#188788

Purpose

Avs1 from Erwinia piriflorinigrans CFBP5888

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EpAvs1

Expression

Bacterial

Promoter

bla

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDM1485

Plasmid

#207446

Purpose

Extrachromosomal expression of GFP-MhcA

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Insert

mhcA

Use

Unspecified

Tags

GFP

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0047

Plasmid

#205139

Purpose

Expression of 6xHis-EGFP-2xPX in bacteria

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_SABV_GPC

Plasmid

#207305

Purpose

Mammalian expression of Sabia virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SABV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_ONNV_E1E2

Plasmid

#207296

Purpose

Mammalian expression of Onyong-nyong virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ONNV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_LCMV_GPC

Plasmid

#207288

Purpose

Mammalian expression of Lymphocytic choriomeningitis virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LCMV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_PICV_GPC

Plasmid

#207298

Purpose

Mammalian expression of Pichinde virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PICV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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