We narrowed to 4,337 results for: Pet2

1. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

   Plasmid

   #208626

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 191~307, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

   Plasmid

   #208627

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 299~398, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

   Plasmid

   #208629

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 212~285, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175788

   Purpose

   Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #175801

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

    Plasmid

    #157674

    Purpose

    Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
    

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid

    #153444

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. M61 pPET28c-Xenopus beta catenin domains R10C

    Plasmid

    #17202

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains R10C

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. M60 pPET28a-Xenopus beta catenin domains NR7

    Plasmid

    #17201

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains NR7

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

    Plasmid

    #111072

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. M57 pPET28a-TEV-full-length human beta catenin

    Plasmid

    #17198

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid

    #90473

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Insert

    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. M58 pPET28b-TEV-full-length zebrafish beta catenin

    Plasmid

    #17199

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1 Zebrafish)
    

    Tags

    HIS

    Expression

    Bacterial

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only