We narrowed to 6,501 results for: nls
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Plasmid#188259PurposePlasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcLYN268 (LYN Human)
UseCRISPRExpressionMammalianAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_[10x 2EBS-S10:35Smp:NLS:YPet:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254064PurposeYPet expression driven by 10x 2EBS-S10DepositorInsert10x 2EBS-S10:35Smp:NLS:YPet:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceAug. 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega2_[EBSn:mini35S:3x SV40-NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254068PurposeGUS expression driven by EBSnDepositorInsertEBSn:mini35S:3x SV40-NLS:GUS:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_[mEBSn:mini35S:3x SV40-NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254070PurposeGUS expression driven by mEBSnDepositorInsertmEBSn:mini35S:3x SV40-NLS:GUS:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega2_[10x 2EBS-S10:35Smp:NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254066PurposeGUS expression driven by 10x 2EBS-S10DepositorInsert10x 2EBS-S10:35Smp:NLS:GUS:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-GFP-he1.1:CFP
Plasmid#184815PurposeUsed to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic lineDepositorInsertQUAS:NLS-GFP-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-GFPAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194890PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1
UseLentiviralTagsHAMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194894PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194886PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
Plasmid#219537Purposea. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotorDepositorAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-TO-MYOD1-shOct4
Plasmid#182309PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationshOct4PromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
Tags8xHIS and BPNLSExpressionBacterialPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG
Plasmid#86871PurposeTo express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSF3B6 (SF3B6 Human)
TagsEGFP and FLAGExpressionMammalianMutationamino acids 7-29 deletionPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-YFP-NLS
Plasmid#145274PurposeMammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCS and YFP
ExpressionMammalianAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDDE488Q-pCDNA3.1(+)
Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH T78A
Plasmid#212997Purposeexpresses human PHGDHDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHGDH (PHGDH Human)
TagsFLAGExpressionMammalianMutationT78APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH R135W
Plasmid#212998Purposeexpresses human PHGDHDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHGDH (PHGDH Human)
TagsFLAGExpressionMammalianMutationR135WPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH V261M
Plasmid#212999Purposeexpresses human PHGDHDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHGDH (PHGDH Human)
TagsFLAGExpressionMammalianMutationV261MPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTY-F
Plasmid#17439PurposeExpression of UTY in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUTY (UTY Human)
Tags6xMyc and FlagExpressionMammalianAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by