Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,859 results for: sgRNA

Clear keywords
Showing: 1921 - 1940 of 8859 results

  1. pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668

    Plasmid
    #238174
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF4 sgRNA CRISPRa (KLF4 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238174-plasmid-map-sequence-id-474465

    Enlarge

  2. pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666

    Plasmid
    #238172
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238172-plasmid-map-sequence-id-474457

    Enlarge

  3. pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665

    Plasmid
    #238173
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238173-plasmid-map-sequence-id-474456

    Enlarge

  4. pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

    Plasmid
    #238177
    Purpose
    Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    HES7 sgRNA CRISPRa (HES7 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238177-plasmid-map-sequence-id-474455

    Enlarge

  5. pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

    Plasmid
    #238187
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF4 sgRNA CRISPRa (KLF4 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    mTagBFP2
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238187-plasmid-map-sequence-id-474450

    Enlarge

  7. pWR63

    Plasmid
    #203331
    Purpose
    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203331-plasmid-map-sequence-id-409751

    Enlarge

  8. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218156-plasmid-map-sequence-id-434901

    Enlarge

  9. pSCBE3-Hypa

    Plasmid
    #218157
    Purpose
    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    218157-plasmid-map-sequence-id-433172

    Enlarge

  12. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

    Enlarge

  16. IABE

    Plasmid
    #247332
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with TadA8e driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-2×TadA8e(V106W)_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247332-plasmid-map-sequence-id-496501

    Enlarge

  17. ICBE

    Plasmid
    #247331
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247331-plasmid-map-sequence-id-496500

    Enlarge

  18. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    73715-plasmid-map-sequence-id-200872

    Enlarge

Showing: 1921 - 1940 of 8859 results