We narrowed to 4,673 results for: pcr
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Plasmid#19690DepositorInserthlh-12(R25K)_genomic region
ExpressionWormAvailable sinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCCM937
Plasmid#58982PurposeExpresses avr-15 sgRNA in C.elegans.DepositorInsertavr-15 sgRNA
UseCRISPRExpressionWormPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCM936
Plasmid#58981PurposeExpresses avr-14 sgRNA in C.elegans.DepositorInsertavr-14 sgRNA
UseCRISPRExpressionWormPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
CX3C1-Tango
Plasmid#66258PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCX3C1 (CX3CR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
ExpressionInsectAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
ExpressionInsectAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0I80_Bxb1mut(noBsaI)_GG1-GG4
Plasmid#219715PurposeLevel 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.DepositorInsertGG1-Bxb1(noBsaI0)-GG4
UseSynthetic BiologyAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-I9 PhiC31 GG1-GG4
Plasmid#195892PurposeLevel 0 PhiC31 integrase with GG1 and GG4 golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma11-GFP2
Plasmid#166780PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma11-GFP2 (GNG11 Human, Synthetic)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-i-pep
Plasmid#47436PurposeFRET sensor for beta2-adrenergic receptorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…ExpressionMammalianPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA5/FRT/TO-GAlphaoB-RLuc8
Plasmid#140977PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphaoB-Rluc8 (GNAO1 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PTGER3-Tango
Plasmid#66485PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEP3-I (PTGER3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-FAID
Plasmid#127885PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID taggingDepositorAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113612PurposePPI reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS1P1
ExpressionMammalianAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL2080 pGATA23 GG0-GG1
Plasmid#195908PurposeLevel 0 pGATA23 promoter with GG0 and GG1 golden gate linkersDepositorInsertpGATA23
UseSynthetic BiologyAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I32 pLBD16 GG0-GG1
Plasmid#195903PurposeLevel 0 pLBD16 promoter with GG0 and GG1 golden gate linkersDepositorInsertpLBD16
UseSynthetic BiologyAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD425
Plasmid#202078PurposeFrame +0 selector for NHEJ knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpEF1a-mCherry-Cas9 + Frame +0 sgRNA
UseCRISPRPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD427
Plasmid#202080PurposeFrame +2/-1 selector for NHEJ knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA
UseCRISPRPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I39 PhiC31-Intein GG1-GG4
Plasmid#195905PurposeLevel 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkersDepositorInsertPhiC31-intein
UseSynthetic BiologyAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only