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Showing: 2941 - 2970 of 3980 results

  8. SOX2-P2A-tagBFP-HDR

    Plasmid
    #163751
    Purpose
    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    P2A-tagBFP
    Expression
    Mutation
    Does not contain start codon to avoid random inte…
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163751-plasmid-map-sequence-id-326711

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  13. pLenti-xF2X-P2A-Puro

    Plasmid
    #110873
    Purpose
    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Article
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xFNLS-2X(3.7)
    Use
    Lentiviral
    Tags
    3X FLAG
    Expression
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110873-plasmid-map-sequence-id-260843

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  15. iMb2-Control-Mosaic (SO273)

    Plasmid
    #99750
    Purpose
    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99750-plasmid-map-sequence-id-443585

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  28. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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Showing: 2941 - 2970 of 3980 results