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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,581 results for: arl

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Showing: 1961 - 1980 of 3581 results

  2. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  7. pGL2_170

    Plasmid
    #199102
    Purpose
    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
    Depositor
    Charlie Gilbert, Nili Ostrov, Cultivarium Tools
    Publication
    Gilbert et al bioRxiv 2023.05.19.541510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT
    Use
    Synthetic Biology
    Available since
    Sept. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199102-plasmid-map-sequence-id-408504

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  12. pET-PesaR-Clux

    Plasmid
    #47658
    Purpose
    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)
    Depositor
    Cynthia Collins
    Publication
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Additional esa box between -35 site and the origi…
    Promoter
    PesaR-C
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. OSCA3.1

    Plasmid
    #136596
    Purpose
    Expresses the mechanosensitive channel OSCA3.1 in mammalian cells
    Depositor
    Ardem Patapoutian
    Publication
    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OSCA3.1 (ERD4 Mustard Weed)
    Tags
    pIRES2-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136596-plasmid-map-sequence-id-273676

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  17. pQFmcs-2H12.D11-scarREF

    Plasmid
    #221184
    Purpose
    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)
    Depositor
    Urs Jenal
    Publication
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen2) and PQ5 (cumate-inducible)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221184-plasmid-map-sequence-id-437178

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  18. t1-435

    Plasmid
    #166578
    Purpose
    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Publication
    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11
    Insert
    Tln1 Head (1-435) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-435 only
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166578-plasmid-map-sequence-id-330255

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  19. pWZ852

    Plasmid
    #208114
    Purpose
    mSwAP-In marker cassette 1 with mScarlet-HygTK
    Depositor
    Jef Boeke
    Publication
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-HygTK
    Tags
    T2A
    Expression
    Bacterial and Mammalian
    Promoter
    EF1alpha
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    208114-plasmid-map-sequence-id-413317

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  20. pFX-mCherry-Rab5

    Plasmid
    #174455
    Purpose
    Express mCherry with early endosome-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Publication
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry with early endosome-targeting sequence
    Tags
    small GTPase (Rab5)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174455-plasmid-map-sequence-id-354803

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Showing: 1961 - 1980 of 3581 results