We narrowed to 6,001 results for: crispr cas9 expression plasmids

1. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS59

   Plasmid

   #215680

   Purpose

   Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS77

   Plasmid

   #215681

   Purpose

   Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP1

   Plasmid

   #166103

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYC1446

   Plasmid

   #158720

   Purpose

   Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CAG-GFPd2

    Plasmid

    #115665

    Purpose

    Piggybac transposon plasmid for destabilized eGFP (GFPd2)

    Depositor

    Jordan Green

    Article

    Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

    Insert

    GFPd2
    

    Use

    Synthetic Biology

    Tags

    Destabilization

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AP582-2

    Plasmid

    #70049

    Purpose

    Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    mCherry::linker::H2B
    

    Use

    Tags

    Ollas and V5

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML107-NET1

    Plasmid

    #232893

    Purpose

    Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    NET1 gRNA (NET1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML107-VRG4

    Plasmid

    #232899

    Purpose

    Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    VRG4 gRNA (VRG4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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