We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. rtTA-sg-CMV51

   Plasmid

   #188484

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. rtTA-sg-mGreenLantern/EGFP

   Plasmid

   #188482

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-GFPd2

   Plasmid

   #115665

   Purpose

   Piggybac transposon plasmid for destabilized eGFP (GFPd2)

   Depositor

   Jordan Green

   Article

   Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

   Insert

   GFPd2
   

   Use

   Synthetic Biology

   Tags

   Destabilization

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AP582-2

   Plasmid

   #70049

   Purpose

   Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   mCherry::linker::H2B
   

   Use

   Tags

   Ollas and V5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pML107-NET1

   Plasmid

   #232893

   Purpose

   Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   NET1 gRNA (NET1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML107-VRG4

   Plasmid

   #232899

   Purpose

   Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   VRG4 gRNA (VRG4 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML104-HIS3-3

   Plasmid

   #232882

   Purpose

   Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   HIS3 gRNA (HIS3 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML104-HIS3-2

   Plasmid

   #232881

   Purpose

   Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   HIS3 gRNA (HIS3 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRRNAcos

    Plasmid

    #78492

    Purpose

    Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKD1 gRNA (BRDN0001148399)

    Plasmid

    #76842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PKD1 gRNA (BRDN0001149263)

    Plasmid

    #76843

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. non-targeting control gRNA (BRDN0001149198)

    Plasmid

    #80248

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge