1. Mouse c-myc (exon I)

   Plasmid

   #8446

   Depositor

   Frederic Mushinski

   Article

   Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

   Insert

   Mouse c-myc (exon I) (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBa.GFP-AP1/beta1

   Plasmid

   #218802

   Purpose

   Mammalian expression o fhuman AP1 b1

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   AP1 b1 (AP1B1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211842

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   Promoter

   6xUAS-35Smini and pUBQ10

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211844

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter

   Plasmid

   #211847

   Purpose

   Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   R181A R185A

   Promoter

   35S

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_ARHGAP42-1/1

   Plasmid

   #91461

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGAP42-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGAP42-1/1 (ARHGAP42 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-Tet-MKK6(DD)-Puro

   Plasmid

   #86094

   Purpose

   Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

   Depositor

   Cindy Miranti

   Article

   Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Serine 207 and Threonine 211 both changed to Aspa…

   Promoter

   CMV/TetO

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CL20_mEGFP_MEGF10_NUP210

    Plasmid

    #205854

    Purpose

    Express mEGFP-tagged fusion protein, MEGF10_NUP210 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    MEGF10_NUP210 (MEGF10, NUP210 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge