Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 1 - 20 of 20 results
  1. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Insert
    Venus
    Use
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pDH3

    Plasmid
    #83789
    Purpose
    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection
    Depositor
    Insert
    CFP
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    F64L, Y66W, N146I, M153T, V163A, N164H mutations …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. Matrix-roGFP

    Plasmid
    #49437
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells
    Depositor
    Insert
    Matrix-roGFP2
    Use
    Adenoviral
    Tags
    Cytochrome c oxidase subunit IV mitochondrial tar…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Availability
    Academic Institutions and Nonprofits only
  7. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Insert
    NLS-2xBFP(4C)
    Use
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Availability
    Academic Institutions and Nonprofits only
  8. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107576
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
  9. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107577
    Purpose
    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    gapdh promoter - B. megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
  10. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
  11. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107582
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fba promoter Bacillus megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 20 results