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8xGTIIC-luciferase

Plasmid

#34615

Purpose

YAP/TAZ-responsive synthetic promoter driving luciferase expression

Depositor

Stefano Piccolo

Article

Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

Insert

synthetic TEAD luciferase reporter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-EGFP

Plasmid

#84041

Purpose

3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS

Plasmid

#20896

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

p53AS (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

5' natural EcoRV and SacI restriction sites …

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
Mix.2-lux

Plasmid

#20893

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 promoter luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Use

Tags

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-tdTomato-cNLS

Plasmid

#138532

Purpose

Lentiviral inducible expression of tdTomato with nuclear localization signal

Depositor

Ana Teixeira, Christopher Grigsby

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

tdTomato

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-mCherry-cNLS

Plasmid

#138531

Purpose

Lentiviral inducible expression of mCherry with nuclear localization signal

Depositor

Ana Teixeira, Christopher Grigsby

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-DsRed-cNLS

Plasmid

#138533

Purpose

Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Ana Teixeira, Christopher Grigsby

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge