We narrowed to 34 results for: bruchas

1. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ef1a-DIO-OMOR-eYFP

   Plasmid

   #66847

   Purpose

   Expresses Opto-MOR in a Cre dependent manner

   Depositor

   Michael Bruchas

   Article

   Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

   Insert

   Opto Mu opioid receptor
   

   Use

   AAV and Cre/Lox

   Tags

   eYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-DIO-PPO-Venus

   Plasmid

   #139504

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV5

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CRY2-CreN

   Plasmid

   #75268

   Purpose

   The second half of the photo-activatable cre recombinase

   Depositor

   Michael Bruchas

   Article

   Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

   Insert

   second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
   

   Use

   AAV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CIBN-CreC

   Plasmid

   #75267

   Purpose

   The first half of the photo-activatable cre recombinase

   Depositor

   Michael Bruchas

   Article

   Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

   Insert

   N-terminal portion of the CIB1 transcription factor
   

   Use

   AAV

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-mScarlet-gamma9

   Plasmid

   #140097

   Purpose

   Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   GNGT2
   

   Tags

   N-terminal mScarlet tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-optoB2-LYY-YFP

    Plasmid

    #75271

    Purpose

    G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    G-protein independent version of optoB2
    

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    point mutations at L72F,Y136G,Y224A

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-CamKII-TRPV1-P2A-mCherry

    Plasmid

    #200829

    Purpose

    Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-fDIO-tdTomato

    Plasmid

    #128434

    Purpose

    Expresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    tdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

    Plasmid

    #171027

    Purpose

    Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1 and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    ChrimsonR is fused to tdTomato

    Promoter

    Ef1a

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Syn-DIO-TRPV1-P2A-mCherry

    Plasmid

    #200831

    Purpose

    In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV_NOPlight1

    Plasmid

    #195578

    Purpose

    Expresses NOPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Syn-TRPV1-P2A-DsRed

    Plasmid

    #200830

    Purpose

    Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-DsRed (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV- δLight

    Plasmid

    #201224

    Purpose

    Express deltaLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    δLight1.3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge