Addgene

Showing: 1 - 9 of 9 results

CKAP5 C5.2 gRNA

Plasmid

#90630

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKAP5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CKAP5 (Guide Designation C5.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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CKAP5 C6.2 gRNA

Plasmid

#90631

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKAP5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CKAP5 (Guide Designation C6.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
TOG_D1

Plasmid

#139112

Purpose

express first domain (1-250) of TOG

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

TOG/CKAP5 (CKAP5 Human)

Use

Tags

Expression

Bacterial

Mutation

1-250

Promoter

Availability

Academic Institutions and Nonprofits only

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MBP-ch-TOG-His6

Plasmid

#69108

Purpose

For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ch-TOGKDP-GFP

Plasmid

#69112

Purpose

For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP-GFP-shch-TOG

Plasmid

#69113

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

Plasmid

#69114

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

L1939A and L1942A amino acid substitutions. Sile…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Promoter

Availability

Academic Institutions and Nonprofits only

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GFP-Tog

Plasmid

#29480

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K19R, E118G, G254R, K1834R

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge