Showing: 21 - 40 of 406 results
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Plasmid#92026PurposemCherry reporter plasmid with sucA TALE binding site in its promoterDepositorInsertmCherry
UseTALENTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailabilityAcademic Institutions and Nonprofits only -
pAC-DELTA
Plasmid#53273PurposeContains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coliDepositorInsertlcyE (LUT2 Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterTrcAvailabilityAcademic Institutions and Nonprofits only -
pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA-At
Plasmid#53288PurposeContains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.DepositorInsertlcyB (LYC Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)PromoterAvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
LUC56 (Luc/UGA/SP)
Plasmid#112147PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258mutated to selenocysteine (U)PromoterAvailabilityAcademic Institutions and Nonprofits only -
p5A0-T
Plasmid#92016PurposeExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterJ23102 Promoter (from Anderson promoter library) …AvailabilityAcademic Institutions and Nonprofits only -
p5A1-T
Plasmid#92017PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5A1-Ti
Plasmid#92018PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219V, C151APromoterAvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5B3-T
Plasmid#92021PurposeExpresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lysA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5C3-T
Plasmid#92022PurposeExpresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting sucA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABEmax7.10HD-BlastR
Plasmid#176477PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABEmax7.10HD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABE8eHD-BlastR
Plasmid#176478PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABE8eHD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 406 results