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We narrowed to 61 results for: deinococcus radiodurans

Showing: 1 - 20 of 61 results

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pRAD1

Plasmid

#47697

Purpose

General cloning vector for use in E. coli or D. radiodurans

Depositor

Mary Lidstrom

Article

Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

repU

Availability

Academic Institutions and Nonprofits only

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pRADZ1

Plasmid

#47698

Purpose

General cloning vector for use in E. coli or D. radiodurans with lacZ

Depositor

Mary Lidstrom

Article

Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

repU

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+)-DR RecR

Plasmid

#12617

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEINO1

Plasmid

#179472

Purpose

Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Inserts

ori
cat

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-DR RecR(K23A/R27A)

Plasmid

#12619

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Use

Tags

His

Expression

Bacterial

Mutation

changed Lysine 23 to Alanine, and Arginine 27 to …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEINO3

Plasmid

#179487

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

TetR/A

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEINO4

Plasmid

#179488

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aadA1

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD5

Plasmid

#197289

Purpose

Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

NptII

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLICER

Plasmid

#197288

Purpose

Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

I-SceI Endonuclease

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only
pAG25-L-IFP-F[1] (clon 3)

Plasmid

#52899

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Vector for homologous recombination

Tags

Expression

Mutation

Promoter

TEF promoter

Availability

Academic Institutions and Nonprofits only
pAG32-L-IFP-F[2] (clon 3)

Plasmid

#52900

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Vector for homologous recombination

Tags

Expression

Mutation

Promoter

TEF promoter

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Availability

Academic Institutions and Nonprofits only
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

Plasmid

#52893

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[2]

Use

Tags

L-IFP-F[2]

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only